PCR recommendations

1.- Use of separate hoods for handling samples and positive controls and the reconstitution and dispensing of the mix. 

2.- Use of filter tips by trained personal.

3.- PCR instruments should be in a dedicated physically separated area from Pre-PCR laboratory.

4.- A common source of contamination are tubes not properly closed in the qPCR cycler. Please make sure tubes are sealed before running PCR. Prevent contamination of the instrument by spills, and never run a reaction with an open or leaking sample lid. Never run a reaction with a seal that is open, loose, punctured, or otherwise damaged because you could contaminate the block, inner lid, and optical system. Never open a PCR tube after PCR. 

5.- Perform routine cleaning of the working surfaces. The use of DNAZap, or equivalent DNA degrading system is highly recommended.

6.- Perform routine cleaning of the PCR cycler.

Please find below recommendations for CFX96, which could be used for other cyclers. In case a contamination issue is suspected, it is recommended to thoroughly clean well by well the block with 1/100 dilution of bleach and rinse with water.

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