Recommended nucleic acid extraction kits

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• PCR recommendations

  1.- Use of separate hoods for handling samples and positive controls and the reconstitution and dispensing of the mix. 2.- Use of filter tips by trained personal. 3.- PCR instruments should be in a dedicated physically separated area from Pre-PCR ...

• Assigning variants with CT>36 RTPCR010

  Generally, if any of the Ct values for the virus targets (FAM, HEX or Texas/ROX) is above 36, the result should not be considered for lineage assignment. If the N target is in the range of >36 Ct <40 the results should be “SARS CoV2 POSITIVE, LINEAGE ...

• Conservation of STI samples for PCR

  In general terms, the following is recommended for PCR analysis of STI samples: they can be stored at 2-8°C for up to 72 hours after collection, or at a temperature between -25°C and -15°C for longer periods of time.

• No amplification of N with amplification of E targets (RTPCR002)

  In the RTPCR002 kit, the lack of amplification of the N target, despite the amplification of the E target, is due to a mutation in the viral RNA at the probe's target sequence. We recently identified several SARS-CoV-2 sequences with a single ...

• E484K mutation (RTPCR010)

  The RTPCR010 reference does not include the Spike E484K mutation for detecting SARS-CoV-2 variants, as it does not provide any additional discrimination of variants when HV69/70 and N501Y are already used. Additionally, including it would limit the ...