Both the LP and LPP formats use white "low profile" opaque tubes that allow fluorescence reading from the top angle, like the ones seen in the following images:
Both formats are compatible with the LightCycler 480, however, a special frame is required for strips.
1.- Use of separate hoods for handling samples and positive controls and the reconstitution and dispensing of the mix. 2.- Use of filter tips by trained personal. 3.- PCR instruments should be in a dedicated physically separated area from Pre-PCR ...
The LightCycler 480 instrument requires color compensation for a 4-plex assay, as outlined in its manual. Color compensation can be applied using a file from a different assay that is using FAM, HEX, Texas Red (ROX), and Cy5. In case no color ...
Generally, if any of the Ct values for the virus targets (FAM, HEX or Texas/ROX) is above 36, the result should not be considered for lineage assignment. If the N target is in the range of >36 Ct <40 the results should be “SARS CoV2 POSITIVE, LINEAGE ...
The duration of the PCR cycles depends on the heating/cooling ramping of each instruments, however in standard qPCR cyclers RTPCR001 takes about 1h 30 min and RTPCR002/RTPCR003 is 1h 45 min.