The duration of the PCR cycles depends on
the heating/cooling ramping of each instruments, however in standard qPCR
cyclers RTPCR001 takes about 1h 30 min and RTPCR002/RTPCR003 is 1h 45 min.
1.- Use of separate hoods for handling samples and positive controls and the reconstitution and dispensing of the mix. 2.- Use of filter tips by trained personal. 3.- PCR instruments should be in a dedicated physically separated area from Pre-PCR ...
In general terms, the following is recommended for PCR analysis of STI samples: they can be stored at 2-8°C for up to 72 hours after collection, or at a temperature between -25°C and -15°C for longer periods of time.
Both the LP and LPP formats use white "low profile" opaque tubes that allow fluorescence reading from the top angle, like the ones seen in the following images: Both formats are compatible with the LightCycler 480, however, a special frame is ...