Results interpretation in the Vaginal Panel Real Time PCR kit (RTPCR005) in VirCom

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• Conservation of STI samples for PCR

  In general terms, the following is recommended for PCR analysis of STI samples: they can be stored at 2-8°C for up to 72 hours after collection, or at a temperature between -25°C and -15°C for longer periods of time.

• RT-PCR format tubes

  Both the LP and LPP formats use white "low profile" opaque tubes that allow fluorescence reading from the top angle, like the ones seen in the following images: Both formats are compatible with the LightCycler 480, however, a special frame is ...

• Assigning variants with CT>36 RTPCR010

  Generally, if any of the Ct values for the virus targets (FAM, HEX or Texas/ROX) is above 36, the result should not be considered for lineage assignment. If the N target is in the range of >36 Ct <40 the results should be “SARS CoV2 POSITIVE, LINEAGE ...

• PCR recommendations

  1.- Use of separate hoods for handling samples and positive controls and the reconstitution and dispensing of the mix. 2.- Use of filter tips by trained personal. 3.- PCR instruments should be in a dedicated physically separated area from Pre-PCR ...

• Duration of PCR

  The duration of the PCR cycles depends on the heating/cooling ramping of each instruments, however in standard qPCR cyclers RTPCR001 takes about 1h 30 min and RTPCR002/RTPCR003 is 1h 45 min.